Impact of brewery wastewater inhibitors in pure and mixed cultures of the yeast Rhodosporidium toruloides NCYC 921 and the microalga Tetradesmus obliquus ACOI 204/07

Brewery wastewater (BWW) is an appealing low-cost substrate for the production of single cell oils by oleaginous microorganisms. However, it may contain inhibitor compounds that affect microbial metabolism. This work investigated, first time, presence potential in primary brewery (PBWW) and secondary (SBWW) pure mixed cultivation yeast Rhodosporidium toruloides NCYC 921 microalga Tetradesmus obliquus ACOI 204/07. Three organic acids (OrgAc) were identified effluents (acetic, propionic butyric acids). Yeast cultivations performed PBWW SBWW order to understand behaviour microorganisms, individually together. Flow cytometry (FC) was used monitor each population during cultivations, study viability throughout all cultivations. The cells cultures grown both severely affected OrgAc confirmed stress results obtained FC. cultures, able develop, levels conditions considerably lower. Only efficient removal observed.